Recombinant Human FGF2 (bFGF, 154 aa)
Carrier-free recombinant human FGF2 (bFGF), E. coli-expressed, ≥98% pure with endotoxin below 0.1 EU/µg. The standard mitogen for stem-cell and primary-culture maintenance.
SKUGF-FGF2-HU
SpeciesHuman
Expressed inE. coli BL21(DE3)
UniProtP09038
Purity≥98%
Endotoxin≤0.1 EU/µg
I Overview
Bioactivity is defined by an ED50 of 0.1–0.6 ng/mL in the BALB/c 3T3 fibroblast proliferation assay. Lyophilized from sterile-filtered PBS, pH 7.4; carrier-free; a 0.1% BSA carrier option is also available.
II Specifications
| Identity | |
| Species | Human |
|---|---|
| Expressed in | E. coli BL21(DE3) |
| UniProt | P09038 |
| Accession | NP_001997.5 |
| Sequence | Ala135-Ser288 (154 aa mature) |
| Physical | |
| Molecular weight | 17.2 (SDS-PAGE)kDa |
| Formulation | Lyophilized from sterile-filtered PBS, pH 7.4; carrier-free; a 0.1% BSA carrier option is also available. |
| Carrier | carrier-free |
| Biological activity | |
| ED50 | 0.1–0.6 ng/mL |
| Assay | BALB/c 3T3 fibroblast proliferation |
| Quality | |
| Purity | ≥98 (SDS-PAGE & RP-HPLC)% |
| Endotoxin | ≤0.1 (LAL)EU/µg |
| Handling | |
| Reconstitution | Reconstitute with the diluent and volume specified on the vial label and lot datasheet. Mix gently; do not vortex. Dilute into assay buffer or medium as needed. |
| Storage | Store the unopened lyophilized vial at the temperature printed on the label and lot datasheet. After reconstitution, aliquot and store as directed; avoid repeated freeze-thaw cycles. |
| Stability | Stability is lot- and formulation-specific. Follow the expiry and reconstituted-storage limits on the vial label and lot datasheet. |
| Grade | Research use only |
IIIStorage & shipping
Storage & shipping
StoreStore the unopened lyophilized vial at the temperature printed on the label and lot datasheet. After reconstitution, aliquot and store as directed; avoid repeated freeze-thaw cycles.ShipBlue-ice (cold pack)Ship from$35 contiguous US baseline; final service chosen at checkoutStabilityStability is lot- and formulation-specific. Follow the expiry and reconstituted-storage limits on the vial label and lot datasheet.
IV Documents
Every lot is released against the published spec and ships with a Certificate of Analysis for that exact lot. Look up a lot CoA →
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