Recombinant Human FGF-9 (208 aa)
Carrier-free recombinant human FGF-9 (glia-activating factor), E. coli-expressed, ≥97% pure with endotoxin below 0.1 EU/µg. A heparin-binding FGFR ligand and mitogen for glial cells, oligodendrocyte progenitors, and fibroblasts.
SKUGF-FGF9-HU
SpeciesHuman
Expressed inE. coli BL21(DE3)
UniProtP31371
Purity≥97%
Endotoxin≤0.1 EU/µg
I Overview
Bioactivity is defined by an ED50 of 1.0–5.0 ng/mL in the murine NIH/3T3 fibroblast proliferation assay. Sterile-filtered (0.2 µm) solution in PBS with 5 mM DTT, pH 7.4. Supplied frozen and carrier-free by default; a 0.1% BSA carrier option is also offered. The DTT keeps the two unpaired cysteines (Cys68, Cys134) reduced, since FGF-9 has no native disulfide bond to hold them.
II Specifications
| Identity | |
| Species | Human |
|---|---|
| Expressed in | E. coli BL21(DE3) |
| UniProt | P31371 |
| Accession | NP_002001.1 |
| Sequence | Met1-Ser208 (208 aa, full-length open reading frame) |
| Physical | |
| Molecular weight | 23.4 (SDS-PAGE)kDa |
| Formulation | Sterile-filtered (0.2 µm) solution in PBS with 5 mM DTT, pH 7.4. Supplied frozen and carrier-free by default; a 0.1% BSA carrier option is also offered. The DTT keeps the two unpaired cysteines (Cys68, Cys134) reduced, since FGF-9 has no native disulfide bond to hold them. |
| Carrier | carrier-free |
| Biological activity | |
| ED50 | 1.0–5.0 ng/mL |
| Assay | murine NIH/3T3 fibroblast proliferation |
| Quality | |
| Purity | ≥97 (SDS-PAGE & RP-HPLC)% |
| Endotoxin | ≤0.1 (LAL)EU/µg |
| Handling | |
| Thaw & dilution | No reconstitution necessary. Thaw on ice, mix gently, and use directly or dilute into your assay buffer. |
| Storage | -80 °C. Aliquot upon receipt to avoid repeated freeze/thaw. |
| Stability | 12 mo at -80 °C; 1 wk at 2-8 °C once thawed. Do not refreeze. |
| Grade | Research use only |
IIIStorage & shipping
Storage & shipping
Store-80 °C. Aliquot upon receipt to avoid repeated freeze/thaw.ShipDry-ice, overnight onlyShip rate$85 per order, contiguous USStability12 mo at -80 °C; 1 wk at 2-8 °C once thawed. Do not refreeze.
IV Documents
Every lot is released against the published spec and ships with a Certificate of Analysis for that exact lot. Look up a lot CoA →
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