Recombinant Human VEGF-165
HEK293-expressed recombinant human VEGF-165, the dominant physiological VEGF-A isoform, ≥95% pure and supplied frozen. The active species is the disulfide-linked homodimer that engages VEGFR-2 in endothelial proliferation and angiogenesis assays.
SKUGF-VEGF165-HU
SpeciesHuman
Expressed inHEK293 cells
UniProtP15692
Purity≥95%
Endotoxin≤0.1 EU/µg
I Overview
Bioactivity is defined by an ED50 of 1–5 ng/mL in the HUVEC proliferation assay. Sterile-filtered solution in PBS, pH 7.4. Supplied frozen; carrier-free (a 0.1% BSA carrier option is also available). Supplied as the folded, disulfide-linked homodimer, so no refolding or dimerization step is needed.
II Specifications
| Identity | |
| Species | Human |
|---|---|
| Expressed in | HEK293 cells |
| UniProt | P15692 |
| Accession | NP_001165097.1 |
| Sequence | Ala27-Arg191 (165 aa per chain, disulfide-linked homodimer) |
| Physical | |
| Molecular weight | 38.3 (calculated, non-reduced homodimer)kDa |
| Formulation | Sterile-filtered solution in PBS, pH 7.4. Supplied frozen; carrier-free (a 0.1% BSA carrier option is also available). Supplied as the folded, disulfide-linked homodimer, so no refolding or dimerization step is needed. |
| Carrier | carrier-free |
| Biological activity | |
| ED50 | 1–5 ng/mL |
| Assay | HUVEC proliferation |
| Quality | |
| Purity | ≥95 (SDS-PAGE & SEC-HPLC)% |
| Endotoxin | ≤0.1 (LAL)EU/µg |
| Handling | |
| Thaw & dilution | Supplied ready to use, no reconstitution required. Thaw on ice, mix gently, and dilute into your assay buffer or medium. |
| Storage | -80 °C. Aliquot on receipt to avoid repeated freeze/thaw. |
| Stability | 12 mo at -80 °C; 1 wk at 2-8 °C once thawed. Do not refreeze. |
| Grade | Research use only |
IIIStorage & shipping
Storage & shipping
Store-80 °C. Aliquot on receipt to avoid repeated freeze/thaw.ShipDry-ice, overnight onlyShip rate$85 per order, contiguous USStability12 mo at -80 °C; 1 wk at 2-8 °C once thawed. Do not refreeze.
IV Documents
Every lot is released against the published spec and ships with a Certificate of Analysis for that exact lot. Look up a lot CoA →
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