Recombinant Human EGF (53 aa)
Carrier-free recombinant human EGF, E. coli-expressed, ≥98% pure with endotoxin below 0.1 EU/µg. The standard supplement for epithelial, organoid, and stem-cell media.
SKUGF-EGF-HU
SpeciesHuman
Expressed inE. coli BL21(DE3)
UniProtP01133
Purity≥98%
Endotoxin≤0.1 EU/µg
I Overview
Bioactivity is defined by an ED50 of 0.1–0.5 ng/mL in the BALB/c 3T3 fibroblast proliferation assay. Sterile-filtered solution in PBS, pH 7.4, no additives. Supplied frozen; carrier-free (a 0.1% BSA carrier option is also available).
II Specifications
| Identity | |
| Species | Human |
|---|---|
| Expressed in | E. coli BL21(DE3) |
| UniProt | P01133 |
| Accession | NP_001954.2 |
| Sequence | Asn971-Arg1023 (53 aa mature, precursor numbering) |
| Physical | |
| Molecular weight | 6.2 (SDS-PAGE)kDa |
| Formulation | Sterile-filtered solution in PBS, pH 7.4, no additives. Supplied frozen; carrier-free (a 0.1% BSA carrier option is also available). |
| Carrier | carrier-free |
| Biological activity | |
| ED50 | 0.1–0.5 ng/mL |
| Assay | BALB/c 3T3 fibroblast proliferation |
| Quality | |
| Purity | ≥98 (SDS-PAGE & RP-HPLC)% |
| Endotoxin | ≤0.1 (LAL)EU/µg |
| Handling | |
| Thaw & dilution | Supplied ready to use, no reconstitution required. Thaw on ice and dilute directly into culture medium. |
| Storage | -80 °C. Aliquot on receipt to avoid repeated freeze/thaw. |
| Stability | 12 mo at -80 °C; 1 wk at 2-8 °C once thawed. Do not refreeze. |
| Grade | Research use only |
IIIStorage & shipping
Storage & shipping
Store-80 °C. Aliquot on receipt to avoid repeated freeze/thaw.ShipDry-ice, overnight onlyShip rate$85 per order, contiguous USStability12 mo at -80 °C; 1 wk at 2-8 °C once thawed. Do not refreeze.
IV Documents
Every lot is released against the published spec and ships with a Certificate of Analysis for that exact lot. Look up a lot CoA →
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