T4 DNA Ligase (400 cohesive-end units/µL)
Recombinant bacteriophage T4 DNA ligase, ATP-dependent, for sticky-end, blunt-end, and nick ligation in cloning and library construction. Supplied and titrated in cohesive-end units, not Weiss units.
SKURG-T4LIG
Enzyme classATP-dependent DNA ligase (EC 6.5.1.1)
Concentration400 cohesive-end units/µL
Storage-20 °C
I Overview
Activity-defined at 400 cohesive-end units/µL. 1 cohesive-end unit gives 50% ligation of HindIII-cut lambda DNA in 30 min at 16 °C in a 20 µL reaction. These are cohesive-end units, not Weiss units, so titrate against this definition rather than converting. Applications include sticky-end ligation, blunt-end ligation, nick sealing, Golden Gate assembly, NGS adapter ligation, library construction.
II Specifications
| Identity | |
| Enzyme class | ATP-dependent DNA ligase (EC 6.5.1.1) |
|---|---|
| Molecular weight | 55.3 (SDS-PAGE)kDa |
| Activity | |
| Concentration | 400cohesive-end units/µL |
| Unit definition | 1 cohesive-end unit gives 50% ligation of HindIII-cut lambda DNA in 30 min at 16 °C in a 20 µL reaction. These are cohesive-end units, not Weiss units, so titrate against this definition rather than converting. |
| Activity assay | cohesive-end ligation of lambda/HindIII fragments, gel-based |
| Thermostable | no |
| Formulation & handling | |
| Supplied buffer | 10x T4 DNA ligase buffer with 10 mM ATP |
| Applications | sticky-end ligation, blunt-end ligation, nick sealing, Golden Gate assembly, NGS adapter ligation, library construction |
| Storage | -20 °C |
IIIStorage & shipping
Storage & shipping
Store-20 °CShipBlue-ice (cold pack)Ship rate$35 per order, contiguous US
IV Documents
Every lot is released against the published spec and ships with a Certificate of Analysis for that exact lot. Look up a lot CoA →
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