High-Fidelity Hot-Start DNA Polymerase with GC Enhancer (2 units/µL)
Hot-start proofreading DNA polymerase for high-GC and long-amplicon PCR. Published single-molecule sequencing puts proofreading archaeal polymerases of this class roughly 30- to 300-fold below Taq in substitution error rate; lots here are released on measured activity, not on a fidelity figure.
SKURG-Q5HF
Enzyme classDNA polymerase, family B, 3'→5' exonuclease (proofreading)
Concentration2 units/µL
Storage-20 °C
I Overview
Activity-defined at 2 units/µL. 1 unit incorporates 10 nmol dNTP into acid-insoluble material in 30 min at 74 °C. Applications include standard PCR, high-GC amplification, long-range PCR, cloning.
II Specifications
| Identity | |
| Enzyme class | DNA polymerase, family B, 3'→5' exonuclease (proofreading) |
|---|---|
| Molecular weight | 98 (SDS-PAGE)kDa |
| Activity | |
| Concentration | 2units/µL |
| Unit definition | 1 unit incorporates 10 nmol dNTP into acid-insoluble material in 30 min at 74 °C. |
| Activity assay | primer extension incorporation assay, activated salmon-sperm DNA template |
| Hot start | yes |
| Thermostable | yes |
| Formulation & handling | |
| Supplied buffer | 5x reaction buffer with 10 mM MgCl2 (2 mM final at 1x); GC enhancer supplied separately |
| Applications | standard PCR, high-GC amplification, long-range PCR, cloning |
| Storage | -20 °C |
IIIStorage & shipping
Storage & shipping
Store-20 °CShipBlue-ice (cold pack)Ship rate$35 per order, contiguous US
IV Documents
Every lot is released against the published spec and ships with a Certificate of Analysis for that exact lot. Look up a lot CoA →
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