SUMO Protease (Ulp1), His-tagged (10 units/µL)
Recombinant yeast Ulp1 catalytic domain that cleaves SUMO fusion tags by structure, not by sequence, so it leaves the native N-terminal residue on your protein. Works from 4 to 30 °C and carries a His tag so the protease can be pulled out on IMAC after the cut.
SKURG-SUMO
Enzyme classcysteine protease, Ulp1 peptidase (EC 3.4.22.68)
Concentration10 units/µL
Storage-20 °C
I Overview
Activity-defined at 10 units/µL. 1 unit cleaves ≥90% of 2 µg of the SUMO-fusion control substrate in 1 h at 30 °C in a 20 µL reaction. Applications include SUMO-tag removal, fusion-protein processing.
II Specifications
| Identity | |
| Enzyme class | cysteine protease, Ulp1 peptidase (EC 3.4.22.68) |
|---|---|
| Molecular weight | 26 (SDS-PAGE)kDa |
| Activity | |
| Concentration | 10units/µL |
| Unit definition | 1 unit cleaves ≥90% of 2 µg of the SUMO-fusion control substrate in 1 h at 30 °C in a 20 µL reaction. |
| Activity assay | cleavage of SUMO-fusion control, SDS-PAGE densitometry |
| Thermostable | no |
| Formulation & handling | |
| Supplied buffer | 25 mM Tris-HCl pH 8.0, 150 mM NaCl, 1 mM DTT, 50% glycerol |
| Applications | SUMO-tag removal, fusion-protein processing |
| Storage | -20 °C |
IIIStorage & shipping
Storage & shipping
Store-20 °CShipDry-ice, overnight onlyShip rate$85 per order, contiguous USHazarddry ice un1845
IV Documents
Every lot is released against the published spec and ships with a Certificate of Analysis for that exact lot. Look up a lot CoA →
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