High-Fidelity Hot-Start Polymerase, Pfu-Sso7d Fusion (2 units/µL)
Pyrococcus-type proofreading polymerase fused to the Sso7d dsDNA-binding domain, which raises processivity and allows short extension times for fast, high-fidelity amplification.
SKURG-PHUS
Enzyme classDNA polymerase, family B, 3'→5' exonuclease (proofreading, Sso7d fusion)
Concentration2 units/µL
Storage-20 °C
I Overview
Activity-defined at 2 units/µL. 1 unit incorporates 10 nmol dNTP into acid-insoluble material in 30 min at 74 °C. Applications include high-fidelity PCR, cloning, site-directed mutagenesis, NGS library prep.
II Specifications
| Identity | |
| Enzyme class | DNA polymerase, family B, 3'→5' exonuclease (proofreading, Sso7d fusion) |
|---|---|
| Molecular weight | 96 (SDS-PAGE)kDa |
| Activity | |
| Concentration | 2units/µL |
| Unit definition | 1 unit incorporates 10 nmol dNTP into acid-insoluble material in 30 min at 74 °C. |
| Activity assay | primer extension incorporation assay, activated salmon-sperm DNA template |
| Hot start | yes |
| Thermostable | yes |
| Formulation & handling | |
| Supplied buffer | 5x HF buffer and 5x GC buffer supplied; MgCl2 included in both buffers |
| Applications | high-fidelity PCR, cloning, site-directed mutagenesis, NGS library prep |
| Storage | -20 °C |
IIIStorage & shipping
Storage & shipping
Store-20 °CShipBlue-ice (cold pack)Ship rate$35 per order, contiguous US
IV Documents
Every lot is released against the published spec and ships with a Certificate of Analysis for that exact lot. Look up a lot CoA →
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