Recombinant Human Activin A
Carrier-free recombinant human Activin A, CHO-expressed and ≥97% pure. The active species is the disulfide-linked homodimer of two inhibin β-A chains, the core supplement for definitive-endoderm and pluripotency protocols.
SKUGF-ACTA-HU
SpeciesHuman
Expressed inCHO (mammalian)
UniProtP08476
Purity≥97%
Endotoxin≤0.1 EU/µg
I Overview
Bioactivity is defined by an ED50 of 2–10 ng/mL in the HepG2 Smad2/3 (CAGA)12 luciferase reporter induction assay. Lyophilized from sterile-filtered PBS, pH 7.4; carrier-free; a 0.1% BSA carrier option is also available. Supplied as the folded, disulfide-linked homodimer, so no refolding or dimerization step is needed.
II Specifications
| Identity | |
| Species | Human |
|---|---|
| Expressed in | CHO (mammalian) |
| UniProt | P08476 |
| Accession | NP_002183.1 |
| Sequence | Gly311-Ser426 (116 aa per chain, disulfide-linked homodimer) |
| Physical | |
| Molecular weight | 26 (SDS-PAGE, non-reduced homodimer)kDa |
| Formulation | Lyophilized from sterile-filtered PBS, pH 7.4; carrier-free; a 0.1% BSA carrier option is also available. Supplied as the folded, disulfide-linked homodimer, so no refolding or dimerization step is needed. |
| Carrier | carrier-free |
| Biological activity | |
| ED50 | 2–10 ng/mL |
| Assay | HepG2 Smad2/3 (CAGA)12 luciferase reporter induction |
| Quality | |
| Purity | ≥97 (SDS-PAGE & RP-HPLC)% |
| Endotoxin | ≤0.1 (LAL)EU/µg |
| Handling | |
| Reconstitution | Reconstitute with the diluent and volume specified on the vial label and lot datasheet. Mix gently; do not vortex. Dilute into assay buffer or medium as needed. |
| Storage | Store the unopened lyophilized vial at the temperature printed on the label and lot datasheet. After reconstitution, aliquot and store as directed; avoid repeated freeze-thaw cycles. |
| Stability | Stability is lot- and formulation-specific. Follow the expiry and reconstituted-storage limits on the vial label and lot datasheet. |
| Grade | Research use only |
IIIStorage & shipping
Storage & shipping
StoreStore the unopened lyophilized vial at the temperature printed on the label and lot datasheet. After reconstitution, aliquot and store as directed; avoid repeated freeze-thaw cycles.ShipBlue-ice (cold pack)Ship from$35 contiguous US baseline; final service chosen at checkoutStabilityStability is lot- and formulation-specific. Follow the expiry and reconstituted-storage limits on the vial label and lot datasheet.
IV Documents
Every lot is released against the published spec and ships with a Certificate of Analysis for that exact lot. Look up a lot CoA →
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