Recombinant Human Activin A
Carrier-free recombinant human Activin A, CHO-expressed and ≥97% pure. The active species is the disulfide-linked homodimer of two inhibin β-A chains, the core supplement for definitive-endoderm and pluripotency protocols.
SKUGF-ACTA-HU
SpeciesHuman
Expressed inCHO (mammalian)
UniProtP08476
Purity≥97%
Endotoxin≤0.1 EU/µg
I Overview
Bioactivity is defined by an ED50 of 2–10 ng/mL in the HepG2 Smad2/3 (CAGA)12 luciferase reporter induction assay. Sterile-filtered solution in PBS, pH 7.4. Supplied frozen; carrier-free (a 0.1% BSA carrier option is also available). Supplied as the folded, disulfide-linked homodimer, so no refolding or dimerization step is needed.
II Specifications
| Identity | |
| Species | Human |
|---|---|
| Expressed in | CHO (mammalian) |
| UniProt | P08476 |
| Accession | NP_002183.1 |
| Sequence | Gly311-Ser426 (116 aa per chain, disulfide-linked homodimer) |
| Physical | |
| Molecular weight | 26 (SDS-PAGE, non-reduced homodimer)kDa |
| Formulation | Sterile-filtered solution in PBS, pH 7.4. Supplied frozen; carrier-free (a 0.1% BSA carrier option is also available). Supplied as the folded, disulfide-linked homodimer, so no refolding or dimerization step is needed. |
| Carrier | carrier-free |
| Biological activity | |
| ED50 | 2–10 ng/mL |
| Assay | HepG2 Smad2/3 (CAGA)12 luciferase reporter induction |
| Quality | |
| Purity | ≥97 (SDS-PAGE & RP-HPLC)% |
| Endotoxin | ≤0.1 (LAL)EU/µg |
| Handling | |
| Thaw & dilution | Supplied ready to use, no reconstitution required. Thaw on ice, mix gently, and dilute into your assay buffer or medium. |
| Storage | -80 °C. Aliquot on receipt to avoid repeated freeze/thaw. |
| Stability | 12 mo at -80 °C; 1 wk at 2-8 °C once thawed. Do not refreeze. |
| Grade | Research use only |
IIIStorage & shipping
Storage & shipping
Store-80 °C. Aliquot on receipt to avoid repeated freeze/thaw.ShipDry-ice, overnight onlyShip rate$85 per order, contiguous USStability12 mo at -80 °C; 1 wk at 2-8 °C once thawed. Do not refreeze.
IV Documents
Every lot is released against the published spec and ships with a Certificate of Analysis for that exact lot. Look up a lot CoA →
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