Ni-NTA Agarose, 6% cross-linked
Nitrilotriacetic acid resin charged with Ni2+ on 6% cross-linked agarose, binding ≥40 mg of 6xHis-tagged protein per mL of resin. The standard IMAC support for His-tag capture under native or denaturing conditions.
SKURS-NINTA
LigandNi2+-NTA (nitrilotriacetic acid)
Matrix6% cross-linked agarose
DBC40 mg 6xHis protein/mL resin
Formatbulk-resin
I Overview
A spherical matrix (6% cross-linked agarose) with a dynamic binding capacity of 40 mg 6xHis protein/mL resin. Supplied as bulk resin.
II Specifications
| Ligand & matrix | |
| Ligand | Ni2+-NTA (nitrilotriacetic acid) |
|---|---|
| Matrix | 6% cross-linked agarose |
| Bead type | spherical |
| Bead size | 90 (d50 volume-weighted)µm |
| Format | bulk-resin |
| Performance | |
| Dynamic binding capacity | 40 (10% breakthrough, 6xHis-GFP model protein)mg 6xHis protein/mL resin |
| Max linear flow | 300cm/hr |
| Max pressure | 0.1MPa |
| Buffers & maintenance | |
| Binding buffer | 50 mM NaH2PO4, 300 mM NaCl, 10 mM imidazole, pH 8.0 |
| Elution buffer | 50 mM NaH2PO4, 300 mM NaCl, 250 mM imidazole, pH 8.0 |
| Storage buffer | 30% ethanol |
| CIP | Strip with 100 mM EDTA, wash with 6 M guanidine-HCl or 0.5 M NaOH while uncharged, then recharge with 100 mM NiSO4 and re-equilibrate. Never apply NaOH to a charged bed: the nickel precipitates as hydroxide in the bead and the resin stops binding while still looking blue. |
IIIStorage & shipping
Storage & shipping
ShipAmbientShip rate$12 per order, contiguous US
IV Documents
Every lot is released against the published spec and ships with a Certificate of Analysis for that exact lot. Look up a lot CoA →