Recombinant Human SCF (Stem Cell Factor, 165 aa)
Carrier-free E. coli-expressed recombinant human soluble SCF (KIT ligand), ≥97% pure and supplied frozen. It supports hematopoietic and mast-cell progenitor expansion; the 18.5 kDa chain self-associates into the noncovalent homodimer that engages KIT.
I Overview
Bioactivity is defined by an ED50 of 1–5 ng/mL in the human TF-1 erythroleukemia cell proliferation assay. Sterile-filtered solution in PBS, pH 7.4. Supplied frozen; carrier-free (a 0.1% BSA carrier option is also available). The active species is a noncovalent homodimer of two 18.5 kDa chains that forms on dilution into aqueous buffer, so no refolding or dimerization step is needed.
II Specifications
| Identity | |
| Species | Human |
|---|---|
| Expressed in | E. coli BL21(DE3) |
| UniProt | P21583 |
| Accession | NP_000890.1 |
| Sequence | Glu26-Ala190 (165 aa soluble mature) |
| Physical | |
| Molecular weight | 18.5 (SDS-PAGE, monomer)kDa |
| Formulation | Sterile-filtered solution in PBS, pH 7.4. Supplied frozen; carrier-free (a 0.1% BSA carrier option is also available). The active species is a noncovalent homodimer of two 18.5 kDa chains that forms on dilution into aqueous buffer, so no refolding or dimerization step is needed. |
| Carrier | carrier-free |
| Biological activity | |
| ED50 | 1–5 ng/mL |
| Assay | human TF-1 erythroleukemia cell proliferation |
| Quality | |
| Purity | ≥97 (SDS-PAGE & RP-HPLC)% |
| Endotoxin | ≤0.1 (LAL)EU/µg |
| Handling | |
| Thaw & dilution | Supplied ready to use, no reconstitution required. Thaw on ice, mix gently, and dilute into your assay buffer or medium. |
| Storage | -80 °C. Aliquot on receipt to avoid repeated freeze/thaw. |
| Stability | 12 mo at -80 °C; 1 wk at 2-8 °C once thawed. Do not refreeze. |
| Grade | Research use only |
IIIStorage & shipping
IV Documents
Every lot is released against the published spec and ships with a Certificate of Analysis for that exact lot. Look up a lot CoA →
