Recombinant Human SCF (Stem Cell Factor, 165 aa)
Lyophilized, carrier-free E. coli-expressed recombinant human soluble SCF (KIT ligand), ≥97% pure. It supports hematopoietic and mast-cell progenitor expansion; the 18.5 kDa chain self-associates into the noncovalent homodimer that engages KIT.
SKUGF-SCF-HU
SpeciesHuman
Expressed inE. coli BL21(DE3)
UniProtP21583
Purity≥97%
Endotoxin≤0.1 EU/µg
I Overview
Bioactivity is defined by an ED50 of 1–5 ng/mL in the human TF-1 erythroleukemia cell proliferation assay. Lyophilized from sterile-filtered PBS, pH 7.4; carrier-free; a 0.1% BSA carrier option is also available. The active species is a noncovalent homodimer of two 18.5 kDa chains that forms on dilution into aqueous buffer, so no refolding or dimerization step is needed.
II Specifications
| Identity | |
| Species | Human |
|---|---|
| Expressed in | E. coli BL21(DE3) |
| UniProt | P21583 |
| Accession | NP_000890.1 |
| Sequence | Glu26-Ala190 (165 aa soluble mature) |
| Physical | |
| Molecular weight | 18.5 (SDS-PAGE, monomer)kDa |
| Formulation | Lyophilized from sterile-filtered PBS, pH 7.4; carrier-free; a 0.1% BSA carrier option is also available. The active species is a noncovalent homodimer of two 18.5 kDa chains that forms on dilution into aqueous buffer, so no refolding or dimerization step is needed. |
| Carrier | carrier-free |
| Biological activity | |
| ED50 | 1–5 ng/mL |
| Assay | human TF-1 erythroleukemia cell proliferation |
| Quality | |
| Purity | ≥97 (SDS-PAGE & RP-HPLC)% |
| Endotoxin | ≤0.1 (LAL)EU/µg |
| Handling | |
| Reconstitution | Reconstitute with the diluent and volume specified on the vial label and lot datasheet. Mix gently; do not vortex. Dilute into assay buffer or medium as needed. |
| Storage | Store the unopened lyophilized vial at the temperature printed on the label and lot datasheet. After reconstitution, aliquot and store as directed; avoid repeated freeze-thaw cycles. |
| Stability | Stability is lot- and formulation-specific. Follow the expiry and reconstituted-storage limits on the vial label and lot datasheet. |
| Grade | Research use only |
IIIStorage & shipping
Storage & shipping
StoreStore the unopened lyophilized vial at the temperature printed on the label and lot datasheet. After reconstitution, aliquot and store as directed; avoid repeated freeze-thaw cycles.ShipBlue-ice (cold pack)Ship from$35 contiguous US baseline; final service chosen at checkoutStabilityStability is lot- and formulation-specific. Follow the expiry and reconstituted-storage limits on the vial label and lot datasheet.
IV Documents
Every lot is released against the published spec and ships with a Certificate of Analysis for that exact lot. Look up a lot CoA →
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