RPMI-1640
RPMI-1640 with L-glutamine, phenol red and sodium bicarbonate.
Overview
RPMI-1640 base. Store at 2–8 °C, protected from light.
Specifications
| Formulation | |
| Base formulation | RPMI-1640 |
|---|---|
| Glucose | 2g/L |
| Sodium bicarbonate | 2g/L |
| L-glutamine | with |
| HEPES | no |
| Phenol red | yes |
| Quality specifications | |
| Endotoxin limit | <0.25 EU/mL |
| pH | 7.0–7.4 pH units |
| Osmolality | 265–300 mOsm/kg |
| Handling | |
| Storage | 2–8 °C, protected from light. |
Formulation
The medium contains free L-glutamine, reduced glutathione and phenol red. Serum and antibiotics are not included. Choose supplements for the cell model and experiment; this basal formulation does not provide a complete culture system for every cell type.
Bicarbonate buffering makes pH responsive to carbon dioxide. Set the culture atmosphere according to the cell protocol and verify pH under those conditions. Phenol red helps reveal a change in pH but does not measure its value.
| Ingredient | Chemical form | Amount |
|---|---|---|
| Glycine | Free amino acid | 10 mg/L |
| L-Arginine | Free base | 200 mg/L |
| L-Asparagine | Monohydrate | 56.82 mg/L |
| L-Aspartic acid | Free acid | 20 mg/L |
| L-Cystine dihydrochloride | Dihydrochloride | 65 mg/L |
| L-Glutamic acid | Free acid | 20 mg/L |
| L-Glutamine | Free amino acid | 300 mg/L |
| L-Histidine | Free base | 15 mg/L |
| trans-4-Hydroxy-L-proline | Free amino acid | 20 mg/L |
| L-Isoleucine | Free amino acid | 50 mg/L |
| L-Leucine | Free amino acid | 50 mg/L |
| L-Lysine hydrochloride | Monohydrochloride | 40 mg/L |
| L-Methionine | Free amino acid | 15 mg/L |
| L-Phenylalanine | Free amino acid | 15 mg/L |
| L-Proline | Free amino acid | 20 mg/L |
| L-Serine | Free amino acid | 30 mg/L |
| L-Threonine | Free amino acid | 20 mg/L |
| L-Tryptophan | Free amino acid | 5 mg/L |
| L-Tyrosine disodium salt | Dihydrate | 29 mg/L |
| L-Valine | Free amino acid | 20 mg/L |
| D-Biotin | Anhydrous | 200 µg/L |
| Choline chloride | Anhydrous-basis material | 3 mg/L |
| D-Calcium pantothenate | Calcium salt; anhydrous-basis assay | 250 µg/L |
| Folic acid | Anhydrous | 1 mg/L |
| Niacinamide (nicotinamide) | Anhydrous | 1 mg/L |
| p-Aminobenzoic acid | Free acid | 1 mg/L |
| Pyridoxine hydrochloride | Hydrochloride | 1 mg/L |
| Riboflavin | Anhydrous | 200 µg/L |
| Thiamine hydrochloride | Hydrochloride | 1 mg/L |
| Cyanocobalamin (vitamin B12) | Cyanocobalamin | 5 µg/L |
| myo-Inositol | Anhydrous | 35 mg/L |
| Calcium nitrate | Ca(NO3)2·4H2O tetrahydrate | 100 mg/L |
| Magnesium sulfate | MgSO4·7H2O heptahydrate | 100 mg/L |
| Potassium chloride | KCl | 400 mg/L |
| Sodium bicarbonate | NaHCO3 | 2 g/L |
| Sodium chloride | NaCl | 6 g/L |
| Sodium phosphate dibasic | Anhydrous Na2HPO4 | 800 mg/L |
| D-Glucose | Anhydrous | 2 g/L |
| Glutathione | Reduced | 1 mg/L |
| Phenol red sodium salt | Monosodium salt | 5 mg/L |
Storage & shipping
Documents
Download sample Certificate of Analysis (PDF)
Certificates of Analysis are linked to recorded shipments and require access to the associated order. Access your order’s certificate →
Questions about this product
AI helps find answers in our published product information. Each answer links to its source. For lot results or an application-specific recommendation, contact us.
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Frequently asked questions
What is in this formulation?
The medium contains free L-glutamine, reduced glutathione and phenol red. Serum and antibiotics are not included. Choose supplements for the cell model and experiment; this basal formulation does not provide a complete culture system for every cell type. Bicarbonate buffering makes pH responsive to carbon dioxide. Set the culture atmosphere according to the cell protocol and verify pH under those conditions. Phenol red helps reveal a change in pH but does not measure its value.
Formulation basisHow should I store it?
Storage: 2–8 °C, protected from light.
StorageWhat bottle sizes and quantities can I buy?
RPMI-1640. Selected SKU: 49894-1. 1 bottle; 500 mL per bottle.
Selected productIs there a lot-specific CoA?
Quality-control testing is described in the product information sheet and sample Certificate of Analysis. Lot certificates report the recorded results for the supplied lot.
Quality and lot recordsWhat is the shelf life?
Quality-control testing is described in the product information sheet and sample Certificate of Analysis. Lot certificates report the recorded results for the supplied lot.
Quality and lot records