DMEM low-glucose
DMEM with 1 g/L glucose, sodium bicarbonate and 1 mM sodium pyruvate, without L-glutamine or phenol red.
Overview
DMEM low-glucose base. Store at 2–8 °C, protected from light.
Specifications
| Formulation | |
| Base formulation | DMEM low-glucose |
|---|---|
| Glucose | 1g/L |
| Sodium bicarbonate | 3.7g/L |
| L-glutamine | without |
| HEPES | no |
| Phenol red | no |
| Quality specifications | |
| Endotoxin limit | <0.25 EU/mL |
| pH | 7.0–7.4 pH units at 20–25 °C |
| Osmolality | 310–340 mOsm/kg |
| Handling | |
| Storage | 2–8 °C, protected from light. |
Formulation
This low-glucose DMEM supplies inorganic salts, amino acids and vitamins for laboratory cell-culture work. Its bicarbonate buffer requires a suitable carbon dioxide environment during culture. The ingredient table lists the nominal additions per final litre.
Select supplements for the cell model and experiment. L-glutamine is absent, allowing its amount and timing of addition to be chosen separately. The supplied medium contains no serum or added growth factors.
| Ingredient | Chemical form | Amount |
|---|---|---|
| Glycine | Free amino acid | 30 mg/L |
| L-Arginine hydrochloride | Monohydrochloride | 84 mg/L |
| L-Cystine dihydrochloride | Dihydrochloride | 63 mg/L |
| L-Histidine hydrochloride | Monohydrochloride monohydrate | 42 mg/L |
| L-Isoleucine | Free amino acid | 105 mg/L |
| L-Leucine | Free amino acid | 105 mg/L |
| L-Lysine hydrochloride | Monohydrochloride | 146 mg/L |
| L-Methionine | Free amino acid | 30 mg/L |
| L-Phenylalanine | Free amino acid | 66 mg/L |
| L-Serine | Free amino acid | 42 mg/L |
| L-Threonine | Free amino acid | 95 mg/L |
| L-Tryptophan | Free amino acid | 16 mg/L |
| L-Tyrosine disodium salt | Dihydrate | 104 mg/L |
| L-Valine | Free amino acid | 94 mg/L |
| Choline chloride | Anhydrous-basis material | 4 mg/L |
| D-Calcium pantothenate | Calcium salt; anhydrous-basis assay | 4 mg/L |
| Folic acid | Anhydrous | 4 mg/L |
| Niacinamide (nicotinamide) | Anhydrous | 4 mg/L |
| Pyridoxine hydrochloride | Hydrochloride | 4 mg/L |
| Riboflavin | Anhydrous | 400 µg/L |
| Thiamine hydrochloride | Hydrochloride | 4 mg/L |
| myo-Inositol | Anhydrous | 7.2 mg/L |
| Calcium chloride | CaCl2·2H2O dihydrate | 264.9 mg/L |
| Iron(III) nitrate | Fe(NO3)3·9H2O nonahydrate | 100 µg/L |
| Magnesium sulfate | MgSO4·7H2O heptahydrate | 200 mg/L |
| Potassium chloride | KCl | 400 mg/L |
| Sodium bicarbonate | NaHCO3 | 3.7 g/L |
| Sodium chloride | NaCl | 6.4 g/L |
| Sodium phosphate monobasic | Anhydrous NaH2PO4 | 108.7 mg/L |
| D-Glucose | Anhydrous | 1 g/L |
| Sodium pyruvate | Anhydrous sodium salt | 110 mg/L |
Storage & shipping
Documents
Download sample Certificate of Analysis (PDF)
Certificates of Analysis are linked to recorded shipments and require access to the associated order. Access your order’s certificate →
Questions about this product
AI helps find answers in our published product information. Each answer links to its source. For lot results or an application-specific recommendation, contact us.
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Frequently asked questions
What is in this formulation?
This low-glucose DMEM supplies inorganic salts, amino acids and vitamins for laboratory cell-culture work. Its bicarbonate buffer requires a suitable carbon dioxide environment during culture. The ingredient table lists the nominal additions per final litre. Select supplements for the cell model and experiment. L-glutamine is absent, allowing its amount and timing of addition to be chosen separately. The supplied medium contains no serum or added growth factors.
Formulation basisHow should I store it?
Storage: 2–8 °C, protected from light.
StorageWhat bottle sizes and quantities can I buy?
DMEM low-glucose. Selected SKU: 12637-1. 1 bottle; 500 mL per bottle.
Selected productIs there a lot-specific CoA?
Quality-control testing is described in the product information sheet and sample Certificate of Analysis. Lot certificates report the recorded results for the supplied lot.
Quality and lot recordsWhat is the shelf life?
Quality-control testing is described in the product information sheet and sample Certificate of Analysis. Lot certificates report the recorded results for the supplied lot.
Quality and lot records