DMEM high-glucose
DMEM with 4.5 g/L glucose, L-glutamine, phenol red, sodium pyruvate and sodium bicarbonate.
Overview
DMEM high-glucose base. Store at 2–8 °C, protected from light.
Specifications
| Formulation | |
| Base formulation | DMEM high-glucose |
|---|---|
| Glucose | 4.5g/L |
| Sodium bicarbonate | 3.7g/L |
| L-glutamine | with |
| HEPES | no |
| Phenol red | yes |
| Quality specifications | |
| Endotoxin limit | <0.25 EU/mL |
| pH | 7.0–7.4 pH units |
| Osmolality | 320–350 mOsm/kg |
| Handling | |
| Storage | 2–8 °C, protected from light. |
Formulation
The glucose concentration distinguishes this formulation from low-glucose DMEM. L-glutamine and pyruvate are already present; neither serum nor antibiotics are part of the formulation. The glutamine is the free amino acid, not a glutamine-containing dipeptide.
Bicarbonate buffering links medium pH to the surrounding carbon dioxide concentration. Use the gas conditions and supplementation specified for your cell model, and check pH after equilibration. Phenol red provides a visual indication of pH changes; it does not replace a pH measurement.
| Ingredient | Chemical form | Amount |
|---|---|---|
| Glycine | Free amino acid | 30 mg/L |
| L-Arginine hydrochloride | Monohydrochloride | 84 mg/L |
| L-Cystine dihydrochloride | Dihydrochloride | 63 mg/L |
| L-Glutamine | Free amino acid | 584 mg/L |
| L-Histidine hydrochloride | Monohydrochloride monohydrate | 42 mg/L |
| L-Isoleucine | Free amino acid | 105 mg/L |
| L-Leucine | Free amino acid | 105 mg/L |
| L-Lysine hydrochloride | Monohydrochloride | 146 mg/L |
| L-Methionine | Free amino acid | 30 mg/L |
| L-Phenylalanine | Free amino acid | 66 mg/L |
| L-Serine | Free amino acid | 42 mg/L |
| L-Threonine | Free amino acid | 95 mg/L |
| L-Tryptophan | Free amino acid | 16 mg/L |
| L-Tyrosine disodium salt | Dihydrate | 104 mg/L |
| L-Valine | Free amino acid | 94 mg/L |
| Choline chloride | Anhydrous-basis material | 4 mg/L |
| D-Calcium pantothenate | Calcium salt; anhydrous-basis assay | 4 mg/L |
| Folic acid | Anhydrous | 4 mg/L |
| Niacinamide (nicotinamide) | Anhydrous | 4 mg/L |
| Pyridoxine hydrochloride | Hydrochloride | 4 mg/L |
| Riboflavin | Anhydrous | 400 µg/L |
| Thiamine hydrochloride | Hydrochloride | 4 mg/L |
| myo-Inositol | Anhydrous | 7.2 mg/L |
| Calcium chloride | CaCl2·2H2O dihydrate | 264.9 mg/L |
| Iron(III) nitrate | Fe(NO3)3·9H2O nonahydrate | 100 µg/L |
| Magnesium sulfate | MgSO4·7H2O heptahydrate | 200 mg/L |
| Potassium chloride | KCl | 400 mg/L |
| Sodium bicarbonate | NaHCO3 | 3.7 g/L |
| Sodium chloride | NaCl | 6.4 g/L |
| Sodium phosphate monobasic | Anhydrous NaH2PO4 | 108.7 mg/L |
| D-Glucose | Anhydrous | 4.5 g/L |
| Phenol red sodium salt | Monosodium salt | 15 mg/L |
| Sodium pyruvate | Anhydrous sodium salt | 110 mg/L |
Storage & shipping
Documents
Download sample Certificate of Analysis (PDF)
Certificates of Analysis are linked to recorded shipments and require access to the associated order. Access your order’s certificate →
Questions about this product
AI helps find answers in our published product information. Each answer links to its source. For lot results or an application-specific recommendation, contact us.
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Frequently asked questions
What is in this formulation?
The glucose concentration distinguishes this formulation from low-glucose DMEM. L-glutamine and pyruvate are already present; neither serum nor antibiotics are part of the formulation. The glutamine is the free amino acid, not a glutamine-containing dipeptide. Bicarbonate buffering links medium pH to the surrounding carbon dioxide concentration. Use the gas conditions and supplementation specified for your cell model, and check pH after equilibration. Phenol red provides a visual indication of pH changes; it does not replace a pH measurement.
Formulation basisHow should I store it?
Storage: 2–8 °C, protected from light.
StorageWhat bottle sizes and quantities can I buy?
DMEM high-glucose. Selected SKU: 58099-1. 1 bottle; 500 mL per bottle.
Selected productIs there a lot-specific CoA?
Quality-control testing is described in the product information sheet and sample Certificate of Analysis. Lot certificates report the recorded results for the supplied lot.
Quality and lot recordsWhat is the shelf life?
Quality-control testing is described in the product information sheet and sample Certificate of Analysis. Lot certificates report the recorded results for the supplied lot.
Quality and lot records