DMEM/F12 + 15 mM HEPES
DMEM/F12 with 15 mM HEPES, L-glutamine, phenol red and sodium bicarbonate.
Overview
DMEM/F12 + 15 mM HEPES base. Store at 2–8 °C, protected from light.
Specifications
| Formulation | |
| Base formulation | DMEM/F12 + 15 mM HEPES |
|---|---|
| Glucose | 3.151g/L |
| Sodium bicarbonate | 1.2g/L |
| L-glutamine | with |
| HEPES | yes |
| Phenol red | yes |
| Quality specifications | |
| Endotoxin limit | <0.25 EU/mL |
| pH | 7.0–7.4 pH units |
| Osmolality | 290–320 mOsm/kg |
| Handling | |
| Storage | 2–8 °C, protected from light. |
Formulation
This DMEM/F12 mixture supplies nutrients and inorganic salts for mammalian cell culture. HEPES provides additional buffering during handling; bicarbonate remains part of the buffer system, so the culture atmosphere still affects pH.
The bottle contains a basal medium without added serum, antibiotics or growth factors. Choose supplements and culture conditions for the cells in use. The ingredient table lists the formulation.
| Ingredient | Chemical form | Amount |
|---|---|---|
| Glycine | Free amino acid | 18.75 mg/L |
| L-Alanine | Free amino acid | 4.45 mg/L |
| L-Arginine hydrochloride | Monohydrochloride | 147.5 mg/L |
| L-Asparagine | Monohydrate | 7.5 mg/L |
| L-Aspartic acid | Free acid | 6.65 mg/L |
| L-Cysteine hydrochloride | Monohydrochloride monohydrate | 17.56 mg/L |
| L-Cystine dihydrochloride | Dihydrochloride | 31.29 mg/L |
| L-Glutamic acid | Free acid | 7.35 mg/L |
| L-Glutamine | Free amino acid | 365 mg/L |
| L-Histidine hydrochloride | Monohydrochloride monohydrate | 31.48 mg/L |
| L-Isoleucine | Free amino acid | 54.47 mg/L |
| L-Leucine | Free amino acid | 59.05 mg/L |
| L-Lysine hydrochloride | Monohydrochloride | 91.25 mg/L |
| L-Methionine | Free amino acid | 17.24 mg/L |
| L-Phenylalanine | Free amino acid | 35.48 mg/L |
| L-Proline | Free amino acid | 17.25 mg/L |
| L-Serine | Free amino acid | 26.25 mg/L |
| L-Threonine | Free amino acid | 53.45 mg/L |
| L-Tryptophan | Free amino acid | 9.02 mg/L |
| L-Tyrosine disodium salt | Dihydrate | 55.79 mg/L |
| L-Valine | Free amino acid | 52.85 mg/L |
| D-Biotin | Anhydrous | 3.5 µg/L |
| Choline chloride | Anhydrous-basis material | 8.98 mg/L |
| D-Calcium pantothenate | Calcium salt; anhydrous-basis assay | 2.24 mg/L |
| Folic acid | Anhydrous | 2.65 mg/L |
| Niacinamide (nicotinamide) | Anhydrous | 2.02 mg/L |
| Pyridoxine hydrochloride | Hydrochloride | 2 mg/L |
| Riboflavin | Anhydrous | 219 µg/L |
| Thiamine hydrochloride | Hydrochloride | 2.17 mg/L |
| Cyanocobalamin (vitamin B12) | Cyanocobalamin | 680 µg/L |
| myo-Inositol | Anhydrous | 12.6 mg/L |
| Calcium chloride | CaCl2·2H2O dihydrate | 154.5 mg/L |
| Copper(II) sulfate | CuSO4·5H2O pentahydrate | 1.3 µg/L |
| Iron(III) nitrate | Fe(NO3)3·9H2O nonahydrate | 50 µg/L |
| Iron(II) sulfate | FeSO4·7H2O heptahydrate | 417 µg/L |
| Magnesium chloride | Anhydrous MgCl2 | 28.64 mg/L |
| Magnesium sulfate | MgSO4·7H2O heptahydrate | 100 mg/L |
| Potassium chloride | KCl | 311.8 mg/L |
| Sodium bicarbonate | NaHCO3 | 1.2 g/L |
| Sodium chloride | NaCl | 6.996 g/L |
| Sodium phosphate dibasic | Anhydrous Na2HPO4 | 71.02 mg/L |
| Sodium phosphate monobasic | Anhydrous NaH2PO4 | 54.34 mg/L |
| Zinc sulfate | ZnSO4·7H2O heptahydrate | 432 µg/L |
| D-Glucose | Anhydrous | 3.151 g/L |
| HEPES | Free acid / zwitterion | 3.575 g/L |
| Hypoxanthine monosodium salt | Anhydrous monosodium salt | 2.39 mg/L |
| Linoleic acid | Free acid (liquid) | 42 µg/L |
| DL-α-Lipoic acid | Racemic free acid | 105 µg/L |
| Phenol red sodium salt | Monosodium salt | 8.1 mg/L |
| Putrescine dihydrochloride | Dihydrochloride | 81 µg/L |
| Sodium pyruvate | Anhydrous sodium salt | 55 mg/L |
| Thymidine | Anhydrous | 365 µg/L |
Storage & shipping
Documents
Download sample Certificate of Analysis (PDF)
Certificates of Analysis are linked to recorded shipments and require access to the associated order. Access your order’s certificate →
Questions about this product
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Frequently asked questions
What is in this formulation?
This DMEM/F12 mixture supplies nutrients and inorganic salts for mammalian cell culture. HEPES provides additional buffering during handling; bicarbonate remains part of the buffer system, so the culture atmosphere still affects pH. The bottle contains a basal medium without added serum, antibiotics or growth factors. Choose supplements and culture conditions for the cells in use. The ingredient table lists the formulation.
Formulation basisHow should I store it?
Storage: 2–8 °C, protected from light.
StorageWhat bottle sizes and quantities can I buy?
DMEM/F12 + 15 mM HEPES. Selected SKU: 16054-1. 1 bottle; 500 mL per bottle.
Selected productIs there a lot-specific CoA?
Quality-control testing is described in the product information sheet and sample Certificate of Analysis. Lot certificates report the recorded results for the supplied lot.
Quality and lot recordsWhat is the shelf life?
Quality-control testing is described in the product information sheet and sample Certificate of Analysis. Lot certificates report the recorded results for the supplied lot.
Quality and lot records